Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:10,000

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1:200-1:2,000

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 405 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 405/host/Goat/)
• [Alexa Fluor 430 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 430/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 514 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 514/host/Goat/)
• [Alexa Fluor 532 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 532/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 700 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 700/host/Goat/)
• [Alexa Fluor 750 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 750/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/APC/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [Biotin-XX](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin-XX/host/Goat/)
• [Cyanine3](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Cyanine3/host/Goat/)
• [Cyanine5 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Cyanine5/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [Pacific Blue](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Pacific Blue/host/Goat/)
• [Pacific Orange](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Pacific Orange/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE/host/Goat/)
• [PE-Alexa Fluor 610](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 610/host/Goat/)
• [PE-Alexa Fluor 647](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 647/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Goat/)
• [Rhodamine Red-X](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine Red-X/host/Goat/)
• [Texas Red](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Texas Red/host/Goat/)
• [Texas Red-X](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Texas Red-X/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

558/572 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896347

Target

IgG

Cross Adsorption

human, mouse, rat

Antibody Form

F(ab`)2 Fragment

Product Specific Information

F(ab`)2 antibody fragments offer several benefits over whole IgGs, due to their smaller size (~110 kD vs. ~ 150 kD) and the absence of most of the Fc region. The smaller size allows for better penetration into tissue sections, resulting in improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells with Fc receptors (such as immune cells) and other Fc interaction-related interference.

F(ab`)2 antibody fragments were obtained by a combination of pepsin digestion and immunoaffinity chromatography to remove residual Fc fragments and undigested whole IgG molecules.

To minimize cross-reactivity, the goat anti-rabbit IgG F(ab`)2 fragments have been cross-adsorbed against human, mouse and rat serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Specificity: This antibody binds to heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody does not bind to human, mouse or rat serum proteins/IgG or rabbit non-immunoglobulin serum proteins.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.