Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Biotin, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a critical role in T-cell development and activation.

Preliminary testing indicates OKT8 does not compete with clones RPA-T8 or HIT8a for binding to human peripheral blood leukocytes, suggesting distinct epitopes.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on normal human peripheral blood cells at ≤0.06 µg/test. A test is defined as staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume. Optimal performance requires titration.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family. It functions as a co-receptor for MHC class I molecules and is predominantly expressed on cytotoxic T cells. CD8 exists as CD8αβ heterodimers or CD8αα homodimers on T cells, NK cells, monocytes, and dendritic cells.

Binding of MHC-I/peptide complexes to CD8 triggers Lck recruitment, leading to lymphokine production, motility, and CTL activation. Activated CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.