Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Biotin, eBioscience
인간 CD8a 항원을 인식하는 OKT8 단클론 항체로서, Biotin으로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, T 세포 활성화 연구에 적합합니다. 고순도 Affinity chromatography 정제, 0.5 mg/mL 농도, 연구용 전용 제품입니다.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 22 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494040 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow Series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a critical role in T-cell development and activation.
Preliminary testing indicates OKT8 does not compete with clones RPA-T8 or HIT8a for binding to human peripheral blood leukocytes, suggesting distinct epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on normal human peripheral blood cells at ≤0.06 µg/test. A test is defined as staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume. Optimal performance requires titration.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family. It functions as a co-receptor for MHC class I molecules and is predominantly expressed on cytotoxic T cells. CD8 exists as CD8αβ heterodimers or CD8αα homodimers on T cells, NK cells, monocytes, and dendritic cells.
Binding of MHC-I/peptide complexes to CD8 triggers Lck recruitment, leading to lymphokine production, motility, and CTL activation. Activated CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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