Thermo Fisher Scientific NRF Polyclonal Antibody
Rabbit polyclonal antibody targeting human NFKB Repressing Factor (NRF). Validated for WB, IHC, IHC-P, and IP applications. Supplied as unconjugated liquid at 1 mg/mL, stored at 4°C. Recommended shelf life of 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 50 to 100 of human NFKB Repressing Factor (NKRF, UniProt ID: O15226-1, antigen length: 690 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
This gene encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NF-κB-responsive genes. The protein localizes predominantly to the nucleolus, with a small fraction found in the nucleoplasm and cytoplasm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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