Thermo Fisher Scientific CD28 Polyclonal Antibody
CD28 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성을 가지며 Protein A로 정제된 고순도 항체입니다. 연구용으로 안정적인 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD28, amino acids 1–120 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein, belonging to the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In mice, CD28 is expressed on all CD4+ and CD8+ T cells in peripheral lymphoid organs and blood, with higher expression on immature thymocytes. CD28 dysfunction is associated with diseases such as mycosis fungoides and Sezary’s Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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