Thermo Fisher Scientific Phospho-alpha B Crystallin (Ser45) Polyclonal Antibody
Phospho-alpha B Crystallin (Ser45) 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성이 있으며, Protein A로 정제된 1 mg/mL 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human alpha B Crystallin around the phosphorylation site of Ser45 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens.
The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest and composed of two primary gene products, alpha-A and alpha-B.
Alpha-Crystallin comprises 40% of total lens protein composition and functions as a chaperone, preventing aggregate formation that may lead to cataract formation.
Phosphorylation of alpha-crystallin occurs in response to cellular stress and may play a role in structural control and protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells of affected individuals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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