Thermo Fisher Scientific WAC Polyclonal Antibody
Rabbit polyclonal antibody recognizing human WAC protein. Validated for WB and IHC (Paraffin). Predicted cross-reactivity with mouse and rat. Supplied as liquid, 0.35 mg/mL concentration. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human WAC. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792822 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (94%), Rat (91%).
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35.
Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination.
WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites.
WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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