Thermo Fisher Scientific IgE Monoclonal Antibody (23G3), FITC, eBioscience
마우스 IgE ε-체인에 특이적인 Rat IgG1 단클론 항체로 FITC 형광표지됨. Flow cytometry 및 ELISA에 사용 가능하며, 488 nm/520 nm 파장에서 검출. 0.5 mg/mL 농도의 액상 형태로, 4°C 암소 보관 권장.
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Thermo Fisher Scientific IgE Monoclonal Antibody (23G3), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | ≤ 1 µg/test | View 6 publications |
| ELISA | 1:5000–1:10000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23G3 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465342 |
Product Specific Information
- Description: The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM.
- Applications Reported: Suitable for ELISA and flow cytometric analysis.
- Applications Tested:
- ELISA: 1:5000–1:10000 dilution
- Flow Cytometry: ≤ 1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Optimize antibody titration for best assay performance.
- Excitation/Emission: 488 nm / 520 nm (Blue Laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with four Ig-like constant domains and two light chains. IgE plays an essential role in type I hypersensitivity reactions (e.g., hay fever, asthma, hives, anaphylactic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of these cells, promoting allergic responses. IgE also contributes to immunity against parasites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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