Thermo Fisher Scientific IgE Monoclonal Antibody (23G3), FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 6 publications 6 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

23G3

Conjugate

FITC FITC FITC

View additional formats

• Biotin
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465342

Product Specific Information

Description: The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM.

Applications Reported: 23G3 has been reported for use in ELISA and flow cytometric analysis.

Applications Tested: This 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000. This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.