Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), FITC, eBioscience
마우스 Sca-1(Ly-6A/E) 항원을 인식하는 D7 단클론 항체로, FITC 형광표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 권장됩니다. 마우스 조혈줄기세포 및 면역세포 분석에 적합합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 8 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 147 publications |
| In vitro Assay (IV) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465332 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 711 / 786
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 recognizes both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells.
Non-activated splenocytes from Ly-6.2 strains (C57BL, SJL, 129, AKR, etc.) show higher frequency of D7-positive cells compared to Ly-6.1 strains (BALB/c, C3H, NZB, etc.). Sca-1 expression increases upon activation regardless of Ly-6 haplotype.
The Ly-6 family is involved in regulation and function of T cell activation, and Sca-1 serves as a key marker for mouse hematopoietic progenitor/stem cell subsets.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test, 100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and serves as a marker for murine HSC.
Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC, and is found in fetal liver, adult bone marrow, peripheral blood, and spleen.
It may play a role in the regulation of B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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