Thermo Fisher Scientific Jagged1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

3-15 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Jagged1 Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Jagged1,uniProtId:Q63722-1,ncbiNodeId:10114,antigenRange:32-1068,antigenLength:1219,antigenImageFileName:PA5-47804_Jagged1_Q63722-1_Goat.svg,antigenImageFileNamePDP:PA5-47804_Jagged1_Q63722-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607338

Product Specific Information

In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.