Thermo Fisher Scientific GFP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 - 0.5 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: H(26) K F S V S G E G E G D A T(39) C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325960

Product Specific Information

PA1-980A detects Green Fluorescent Protein (GFP).

PA1-980A has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects recombinant GFP.

PA1-980A immunizing peptide corresponds to the N-terminal amino acid residues 26-39 of Aequorea victoria wild-type GFP. This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. PA1-980A immunizing peptide (Cat. # PEP-033) is available for use in neutralization and control experiments.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.