Thermo Fisher Scientific CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (LPFS2, 4177)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature (RT).
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, including CD40 and CD30. TNF superfamily members regulate cell proliferation and death.
Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells.
Activation of T cells via the TCR-CD3 complex upregulates CD27 expression and releases its soluble form (sCD27), found in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies.
CD27-binding protein (SIVA) is a proapoptotic protein that interacts with CD27 and is involved in apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.