Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1798)
Ep-CAM/CD326 단백질의 세포외 도메인을 인식하는 마우스 단클론 항체로, 인간, 개, 고양이 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합하며, 고순도 Protein A/G 정제액 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) Monoclonal Antibody (EGP40/1798)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| ELISA | 2–4 µg/mL |
| Peptide Array | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1798 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing c-myc and cyclins A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It is localized on the baso-lateral surface of most simple epithelia and carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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