Thermo Fisher Scientific THRB Monoclonal Antibody (J52)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 5 publications 5 publications

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

J52

Immunogen

Purified human TR beta -1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2287303

Product Specific Information

MA1-216 detects thyroid hormone receptor (TR) beta-1 in human, mouse, rat, and transformed bacterial cells. This antibody is specific for the TR beta-1 isoform.

MA1-216 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects a 52 and a 55 kDa protein representing recombinant TR expressed in E. coli. The two different sizes represent two different translational starting points. Immunocytochemical staining of TR beta-1 in intact GH3 cells with MA1-216 results in nuclear staining. When levels of expression of the transfected receptor are high, cytoplasmic staining is seen along with nuclear staining. Immunoprecipitation and gel super shift experiments show that MA1-216 reacts with the unliganded, liganded, and DNA binding forms of the receptor.

The MA1-216 immunogen is purified human TR beta-1 overexpressed in E. coli. This antibody recognizes an epitope in the A/B domain of human TR beta-1, amino acid residues 1-101.

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.