Thermo Fisher Scientific LDHA Recombinant Rabbit Monoclonal Antibody (4H18L6)
LDHA 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 면역세포염색(ICC/IF)에 적합합니다. 인간 LDHA 펩타이드(aa2–19, aa211–229)를 면역원으로 제작되었으며, 높은 특이성과 로트 간 일관성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H18L6 |
| Immunogen | Peptides corresponding to human LDHA (aa211–aa229, aa2–aa19) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2724588 |
Additional Formats
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that catalyzes the conversion of pyruvate to lactate with interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on M and H chain combinations in the tetrameric structure.
LDHA is hypoxia-inducible and regulated by HIF1α, while LDHB is often upregulated in cancers.
Mutations in the LDHA gene can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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