Thermo Fisher Scientific RIP2 Monoclonal Antibody (AF28D3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

1:250

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

3 µL

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AF28D3

Immunogen

Recombinant human protein purified from E.coli (His-RIP2)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538689

Product Specific Information

A suggested positive control for this product is HeLa, 293T, L929 or C6.

Target Information

RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes Lys-63-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces Lys-63-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.