Thermo Fisher Scientific Phospho-Rad17 (Ser645) Polyclonal Antibody
Phospho-Rad17 (Ser645) 폴리클로날 항체로, Western blot 및 IHC 분석에 적합합니다. 인간 및 마우스 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 성장과 DNA 손상 반응 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide representing a portion of human Rad17 around serine 645 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan.
Target Information
Rad17 is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage.
It exhibits weak ATPase activity required for binding to chromatin and participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, as well as in CHEK1 activation.
Rad17 may also serve as a sensor of DNA replication progression and be involved in homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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