Thermo Fisher Scientific PARP12 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human PARP12 (NP_0735871) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP12,uniProtId:Q9H0J9-1,ncbiNodeId:9606,antigenRange:462-701,antigenLength:701,antigenImageFileName:PA5-89749_PARP12_Q9H0J9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89749_PARP12_Q9H0J9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.84 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805758

Product Specific Information

Immunogen sequence: YVSPQDVTTM QTCNTKFPGP KSIPDYWDSS ALPDPGFQKI TLSSSSEEYQ KVWNLFNRTL PFYFVQKIER VQNLALWEVY QWQKGQMQKQ NGGKAVDERQ LFHGTSAIFV DAICQQNFDW RVCGVHGTSY GKGSYFARDA AYSHHYSKSD TQTHTMFLAR VLVGEFVRGN ASFVRPPAKE GWSNAFYDSC VNSVSDPSIF VIFEKHQVYP EYVIQYTTSS KPSVTPSILL ALGSLFSSRQ; Positive Samples: HT-29, HeLa, Mouse brain, Mouse liver, Mouse pancreas, Rat brain; Cellular Location: Nucleus

Target Information

Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.