Thermo Fisher Scientific Enterovirus 71 3D Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of Enterovirus EV71 3D (B9VUU3).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.993 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914346

Product Specific Information

Positive test controls include: HeLa.

Immunogen sequence: MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI IVGYGEWPSY CSDSDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAVLPEYV IGTVAGGTGT EDSHPPY

Target Information

EV71 is a positive-sense single-stranded RNA virus of the Picornaviridae family. It is known to cause hand, foot, and mouth disease and various neurological pathological conditions, such as aseptic meningitis and ataxia. EV71 is shown to utilize host UDP-glucose glycoprotein glucosyltransferase 1 (UGGT1) to enhance viral replication and virulence. It forms replication complexes (RCs) on cellular membranes that contain a mix of host and viral proteins to facilitate viral replication. During infection viral RNA replication occurs on the cytoplasmic surfaces of single-membrane vesicles derived from the endoplasmic reticulum, and the membranes can serve to accelerate RC assembly during positive-strand genomic RNA replication. It has been shown that 3D polymerase, an RNA-dependent RNA polymerase that is encoded in the P3 viral genome region, plays a key role in EVA71 replication. Neural progenitor cells are shown to be prone to EV71 infection and that these stem cells can support the active replication of this virus.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.