Thermo Fisher Scientific Bu-1a Monoclonal Antibody (21-1A4), Biotin
Biotin conjugated Bu-1a monoclonal antibody for chicken B-cell marker detection. Validated for flow cytometry. Mouse IgG1, purified, 0.5 mg/mL in PBS with stabilizer. For research use only.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 1 µg/10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 21-1A4 |
| Immunogen | Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with proprietary stabilizer |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745711 |
Additional Formats
Target Information
Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer.
The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens.
The antibody also identifies B cells in partially inbred birds. Bu-1 is found on:
- 85–90% of bursal cells
- 2–8% of thymocytes
- 15–27% of spleen cells
- 2–18% of peripheral blood cells
It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes.
The monoclonal antibody AV20 does not cross-react with turkey cells when analyzed by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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