Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12) Polyclonal Antibody
히스톤 H4의 아세틸화된 라이신(K5, K8, K12)을 인식하는 Rabbit Polyclonal 항체입니다. Western blot, ICC/IF, ELISA, ChIP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ELISA | 1:1,000 |
| ChIP assay (ChIP) | 1.5 µg/1x10^6 cells |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
| Peptide Array (Array) | 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to human histone H4 region containing acetylated lysines 5, 8, and 12 (H4K5,8,12ac) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608361 |
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of eukaryotic chromatin. Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The histone H4 gene is intronless, lacks polyA tails, and contains a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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