Thermo Fisher Scientific PRKAR1B Monoclonal Antibody (OTI1B4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses such as proliferation, ion transport, metabolism regulation, and gene transcription through activation of cAMP-dependent protein kinase (PKA).
Activation occurs when cAMP binds to the regulatory subunits of the tetrameric PKA holoenzyme, releasing active catalytic subunits.
Elevated cAMP levels lead to translocation of PKA to the nucleus, where it phosphorylates CREB on serine 133, enabling TFIIB binding to TBP1 and linking phospho-CREB to the RNA polymerase II transcription initiation complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.