Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-eFluor 610, eBioscience
인간 CD107a(LAMP-1)을 인식하는 단클론 항체로, PE-eFluor 610 형광체가 결합되어 있습니다. 세포 내 염색 및 유세포분석(Flow Cytometry)에 적합하며, 488–561 nm 레이저로 여기 가능합니다. 광산화에 민감하므로 어둡게 보관하십시오.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574572 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated protein (~110 kDa) primarily located in lysosomal/endosomal membranes. It transiently appears on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and some cancer cells.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
- Recommended use: 5 µL (0.25 µg) per test in 100 µL total volume.
- Suitable for 10⁵–10⁸ cells/test (optimize empirically).
- Excitation: 488–561 nm; Emission: 607 nm.
- Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
- Light sensitive: protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333) for up to 3 days at 4°C in the dark.
- Filtered through 0.2 µm post-manufacturing.
Target Information
LAMP1 (CD107a) is a major lysosomal membrane protein, together with LAMP2, comprising a significant portion of the lysosomal membrane. It plays a key role in lysosome biogenesis and fusion with phagosomes. LAMP1 is a type I integral membrane protein with 18 N-linked glycosylation sites and a short cytosolic tail.
- Involved in lysosome–endosome transport and plasma membrane translocation upon activation.
- Surface LAMP1 promotes PBMC adhesion to vascular endothelium, suggesting a role in inflammation.
- Increased expression observed in neurons and glial cells near senile plaques in Alzheimer’s disease.
- Marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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