Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-eFluor 610, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated protein (~110 kDa) primarily located in lysosomal/endosomal membranes. It transiently appears on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and some cancer cells.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).

• Recommended use: 5 µL (0.25 µg) per test in 100 µL total volume.
• Suitable for 10⁵–10⁸ cells/test (optimize empirically).
• Excitation: 488–561 nm; Emission: 607 nm.
• Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
• Light sensitive: protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333) for up to 3 days at 4°C in the dark.
• Filtered through 0.2 µm post-manufacturing.

Target Information

LAMP1 (CD107a) is a major lysosomal membrane protein, together with LAMP2, comprising a significant portion of the lysosomal membrane. It plays a key role in lysosome biogenesis and fusion with phagosomes. LAMP1 is a type I integral membrane protein with 18 N-linked glycosylation sites and a short cytosolic tail.

• Involved in lysosome–endosome transport and plasma membrane translocation upon activation.
• Surface LAMP1 promotes PBMC adhesion to vascular endothelium, suggesting a role in inflammation.
• Increased expression observed in neurons and glial cells near senile plaques in Alzheimer’s disease.
• Marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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