Thermo Fisher Scientific MCM3 Polyclonal Antibody
MCM3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, IP, ChIP 등에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제되었으며, 4°C 단기 보관 또는 -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 418–657 of Human MCM3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.71 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546582 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (95%), Rat (94%), Zebrafish (80%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The MCM2–7 hexameric complex is a key component of the pre-replication complex (pre_RC) and participates in replication fork formation and recruitment of other DNA replication-related proteins.
MCM3 interacts with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. Acetylation inhibits initiation of DNA replication and cell cycle progression.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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