Thermo Fisher Scientific Cytokeratin 18 (KRT18) Monoclonal Antibody (C-04 (Ks18.04))
다양한 종에서 반응하는 Cytokeratin 18 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 비결합형 액상 항체이며, 단백질 A/G로 정제됨. 인간 및 여러 포유류 조직의 비편평 상피세포 마커로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Cat, Ferret, Goat, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C-04 (Ks18.04) |
| Immunogen | Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Chicken or Xenopus.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8. It is expressed in tissues from the gastrointestinal tract, respiratory tract, urogenital tract, endocrine and exocrine tissues, and mesothelial cells. Cytokeratin 18 is found primarily in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are increasingly used to distinguish different types of epithelial malignancies. Cytokeratin antibodies are useful in the differential diagnosis of tumors using immunohistochemistry, cytopathology, and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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