Thermo Fisher Scientific Cytokeratin 18 (KRT18) Monoclonal Antibody (C-04 (Ks18.04))
Cytokeratin 18 단백질을 인식하는 mouse monoclonal antibody로, 다양한 종에서 반응하며 WB, IHC, ICC, Flow Cytometry에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, -20°C 보관. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Cat, Ferret, Goat, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C-04 (Ks18.04) |
| Immunogen | Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Chicken or Xenopus.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8.
It is expressed in tissues from the gastrointestinal, respiratory, and urogenital tracts, as well as endocrine and exocrine tissues and mesothelial cells.
Cytokeratin 18 is primarily found in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified.
Cytokeratin subtype expression patterns are increasingly used to distinguish different epithelial malignancies.
Cytokeratin antibodies assist in differential tumor diagnosis using immunohistochemistry on tissue sections and are also useful in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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