Thermo Fisher Scientific Phospho-POLR2A (Ser2) Recombinant Rabbit Monoclonal Antibody (ARC1540)
Phospho-POLR2A (Ser2) 인산화 특이 항체로, Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 발현 시스템에서 생산된 Rabbit Recombinant Monoclonal 형식. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg of chromatin |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
| CUT&RUN (C&R) | 10⁵ cells / 1 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1540 |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding Ser2 of human POLR2A CTD repeat YSPTSPS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898146 |
Product Specific Information
Positive Samples: MCF7, HeLa, C2C12, C6
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR1A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR1A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center with the second largest subunit and is part of the core element that includes the central cleft, clamp, and jaws that interact with DNA.
During transcription initiation, the single-stranded DNA template is positioned within the active site cleft of Pol II. The RPB1 subunit contributes a bridging helix that promotes translocation of Pol II by acting as a ratchet mechanism. Transcription elongation is modulated by phosphorylation of the C-terminal domain (CTD) of RPB1, which serves as a platform for assembly of regulatory factors involved in initiation, elongation, termination, and mRNA processing. The balance between methylation and acetylation of CTD lysines is critical for gene expression regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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