Thermo Fisher Scientific ECS (DYKDDDDK) Tag Recombinant Rabbit Monoclonal Antibody (BLRE00G)
DYKDDDDK(FALG) 태그 단백질 검출용 항체로 다양한 응용(WB, ICC/IF, ELISA, IP)에 적합. 재조합 단일클론 토끼 IgG 항체로 높은 특이성과 재현성 제공. HEK293 발현 시스템 기반으로 정제된 액상 형태. 연구용 단백질 정량 및 위치 분석에 최적화.
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Thermo Fisher Scientific ECS (DYKDDDDK) Tag Recombinant Rabbit Monoclonal Antibody (BLRE00G)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 – 1:5,000 |
| ELISA | 1:1,000 – 1:75,000 |
| Immunoprecipitation (IP) | 20 µL/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLRE00G |
| Immunogen | Peptide representing ECS, Enterokinase Cleavage Site (DYKDDDDK) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
It is often fused to the N- or C-terminus of a protein of interest, allowing easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research for protein-protein interaction studies, expression analysis, and localization studies.
Its hydrophilic nature minimizes protein denaturation or inactivation, improving compatibility with attached proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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