Thermo Fisher Scientific MLK4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MLK4. Recombinant protein control fragment (Product #RP-89790). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MLK4,uniProtId:Q5TCX8-1,ncbiNodeId:9606,antigenRange:897-1033,antigenLength:1036,antigenImageFileName:PA5-52460_MLK4_Q5TCX8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52460_MLK4_Q5TCX8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644003

Product Specific Information

Immunogen sequence: GNPTPTGATI ISATGASALP LCPSPAPHSH LPREVSPKKH STVHIVPQRR PASLRSRSDL PQAYPQTAVS QLAQTACVVG RPGPHPTQFL AAKERTKSHV PSLLDADVEG QSRDYTVPLC RMRSKTSRPS IYELEKE

Highest antigen sequence identity to the following orthologs: Mouse - 33%, Rat - 34%.

Target Information

MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4 and does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.