Thermo Fisher Scientific DDX1 Polyclonal Antibody
DDX1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제 제품으로 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 294–608 of Human DDX1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545686 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2
- Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (89%), Xenopus laevis (88%), Chicken (93%), Rhesus Monkey (98%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase capable of unwinding both RNA-RNA and RNA-DNA duplexes. It possesses 5′ single-stranded RNA overhang nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, serves as a coactivator to enhance NF-kappa-B-mediated transcriptional activation and positively regulates cyclin CCND2 expression by binding to its promoter region. It is also part of a multi-helicase-TICAM1 complex involved in sensing cytoplasmic viral double-stranded RNA, triggering antiviral responses and proinflammatory cytokine induction via TICAM1.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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