Thermo Fisher Scientific SMAD3 Polyclonal Antibody
SMAD3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP에 사용 가능. Human, Mouse, Rat에 반응하며 고순도 항원 친화 크로마토그래피로 정제됨. 세포 기반 형광 이미징 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| Immunoprecipitation (IP) | 1:25 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a central region unique to Smad3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10981224 |
Product Specific Information
Antibodies to this protein were previously included in the Thermo Scientific Cellomics High Content Screening Kit.
This replacement antibody is recommended for researchers performing high content cell-based assays.
It has been validated for cellular immunofluorescence (IF) applications.
Further optimization, including the choice of appropriate DyLight-conjugated secondary antibodies, may be required for specific assays.
It is not recommended to aliquot this antibody.
Target Information
SMAD3 belongs to the SMAD family of proteins, homologous to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and is implicated in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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