Thermo Fisher Scientific BOB.1 Monoclonal Antibody (BOB1, 2425)
Human BOB1 단백질을 표적으로 하는 마우스 단클론 항체로, WB 및 IHC(P) 등에 사용 가능. 고순도 Protein A/G 정제, 200 µg/mL 농도. PBS 기반 완충액에 안정화되어 연구용으로 적합.
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- 5450-MSM5-P1
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Applications
- Western Blot (WB): 1–2 µg/mL
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
- Peptide Array (Array): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BOB1, 2425 |
| Immunogen | Recombinant fragment (around aa 148–255) of human BOB1 (POU2AF1) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Transcriptional coactivator that specifically associates with either OCT1 or OCT2.
It boosts OCT1-mediated promoter activity and, to a lesser extent, that of OCT2.
It has no intrinsic DNA-binding activity and recognizes the POU domains of OCT1 and OCT2.
Essential for B-cell response to antigens and required for germinal center formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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