Thermo Fisher Scientific BOB.1 Monoclonal Antibody (BOB1, 2424)
Human BOB1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. Recombinant fragment(aa 148-255)을 면역원으로 사용하며, Protein A/G 정제 및 PBS buffer 보관. 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BOB1, 2424 |
| Immunogen | Recombinant fragment (around aa 148–255) of human BOB1 (POU2AF1) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Transcriptional coactivator that specifically associates with either OCT1 or OCT2.
It boosts OCT1-mediated promoter activity and, to a lesser extent, that of OCT2.
It has no intrinsic DNA-binding activity and recognizes the POU domains of OCT1 and OCT2.
Essential for the response of B-cells to antigens and required for the formation of germinal centers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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