Thermo Fisher Scientific IMPACT Polyclonal Antibody
IMPACT 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간 및 생쥐 반응성 확인. WB, IHC, ICC 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태로 안정적 보관 가능.
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Thermo Fisher Scientific IMPACT Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human IMPACT. Recombinant protein control fragment (Product #RP-98248). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642711 |
Product Specific Information
Immunogen sequence:
ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVV
Highest antigen sequence identity to the following orthologs:
- Mouse: 98%
- Rat: 98%
Target Information
Translational regulator that ensures constant high levels of translation under various stress conditions such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment, and glucose deprivation. Acts as a negative regulator of EIF2AK4/GCN2 kinase activity by impairing GCN1-mediated activation, thus reducing eIF-2-alpha phosphorylation and down-regulation of protein synthesis. May regulate translation in specific neuronal cells under amino acid starvation by preventing GCN2 activation and ATF4 synthesis. Through inhibition of EIF2AK4/GCN2, it contributes to neuronal cell differentiation and neurite outgrowth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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