Thermo Fisher Scientific TOP2A Monoclonal Antibody (A3F12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A3F12

Immunogen

Synthetic peptide within human Topoisomerase II alpha aa 1480-1531/1531. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TOP2A,uniProtId:P11388-1,ncbiNodeId:9606,antigenRange:1480-1531,antigenLength:1531,antigenImageFileName:MA5-50579_TOP2A_P11388-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50579_TOP2A_P11388-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein G

Storage buffer

TBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093395

Product Specific Information

Positive control: A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue.

Predicted band size: 174/178/182 kDa

Subcellular Location: Cytoplasm, Nucleus.

Target Information

Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.