Thermo Fisher Scientific FL - RIP140L8 Peptide, 100 μM

Thermo Fisher Scientific FL - RIP140L8 Peptide, 100 μM

Thermo Scientific™ LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How They Work

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events including:

• Dissociation of repressor proteins
• Association of coactivator proteins
• Assembly of pol II and other transcription factors for activation of target genes

TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding.
These assays can determine the affinity of ligand-bound receptors for different coregulator peptides, or identify additional agonists or antagonists via displacement or recruitment of specific coregulator peptides.

In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody indirectly labels GST-tagged RXR beta protein.
An agonist (9-cis Retinoic Acid) binds to the receptor, causing a conformational change that recruits the Fl-D22 coactivator peptide.
The binding of the fluorescent coregulator peptide to RXR beta results in an increase in the TR-FRET emission ratio.

Contents and Storage

LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers.
Store peptides at -20°C.

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