Thermo Fisher Scientific FL-AR N-TERM Peptide

Thermo Fisher Scientific FL-AR N-TERM Peptide

Thermo Scientific™ LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How They Work

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events including:

• Dissociation of repressor proteins
• Association of coactivator proteins
• Assembly of pol II and other transcriptional factors for activation of target genes

TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding. This can be done by:

1. Determining the affinity of ligand-bound receptor for different coregulator peptides
2. Identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide

In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GST-tagged RXR beta protein. An agonist (9-cis Retinoic Acid) binds to the receptor, causing a conformational change that results in recruitment of the Fl-D22 coactivator peptide. The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio.

Contents and Storage

LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers.
Store peptides at -20°C.

Specifications