Thermo Fisher Scientific Choline Oxidase Polyclonal Antibody
Choline Oxidase를 인식하는 염소 유래 Polyclonal 항체로 Western blot, ELISA, IP에 사용 가능. Alcaligenes species 유래 항원으로 제작되었으며, 고순도 정제 및 안정한 저장 조건 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Choline Oxidase [Alcaligenes species] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross-reactivity against Choline Oxidase from other sources is unknown.
Target Information
Choline Oxidase catalyzes the reaction:
Choline + 2 O₂ + H₂O → Betaine + 2 H₂O₂
This enzyme is involved in the first step of the subpathway that synthesizes betaine from choline.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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