Thermo Fisher Scientific I-Ab/I-Ad Monoclonal Antibody (25-9-17S), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

25-9-17S

Immunogen

C3H.SW splenocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P01921-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P01921-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P01921-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P06342-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P06342-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P06342-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P06343-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P06343-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P06343-1_House_mouse_PDP.jpeg,sortOrder:3},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P06344-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P06344-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P06344-1_House_mouse_PDP.jpeg,sortOrder:4},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P06345-1,ncbiNodeId:10090,antigenRange:1-263,antigenLength:263,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P06345-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P06345-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P06346-1,ncbiNodeId:10090,antigenRange:1-252,antigenLength:252,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P06346-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P06346-1_House_mouse_PDP.jpeg,sortOrder:6},{targetFamily:MHC Class II (I-Ab/I-Ad),uniProtId:P14483-1,ncbiNodeId:10090,antigenRange:1-265,antigenLength:265,antigenImageFileName:MA5-17777_MHC_Class_II_I-AbI-Ad_P14483-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17777_MHC_Class_II_I-AbI-Ad_P14483-1_House_mouse_PDP.jpeg,sortOrder:7}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Biotin
• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539161

Product Specific Information

MA5-17777 targets I-Ab/I-Ad in FACS applications and shows reactivity with Mouse samples.

The MA5-17777 immunogen is c3H.SW splenocytes.

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.