Thermo Fisher Scientific Carbonic Anhydrase IX Recombinant Rabbit Monoclonal Antibody (4F12)
인간 CA9 단백질을 인식하는 재조합 토끼 단일클론 항체로, IHC(P) 및 ELISA에 적합합니다. HEK293 세포에서 발현되며, 고순도 친화 크로마토그래피로 정제되었습니다. 비결합형 액상 형태로 제공되며, 저온(-20°C 이하)에서 안정적으로 보관 가능합니다.
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Thermo Fisher Scientific Carbonic Anhydrase IX Recombinant Rabbit Monoclonal Antibody (4F12)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4F12 |
| Immunogen | A synthesized peptide derived from human CA9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3092778 |
Target Information
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1 alpha, due to its stability and membrane localization. Carbonic anhydrases play a key role in pH regulation by controlling hydrogen ion flux.
Under hypoxic conditions, inhibition of CA IX increases cell death, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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