Thermo Fisher Scientific Phospho-SKI (Ser383) Polyclonal Antibody
Phospho-SKI (Ser383) 폴리클로날 항체로 인산화된 SKI 단백질을 특이적으로 검출. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 정제 및 안정적 액상 형태로 연구용에 최적화.
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Thermo Fisher Scientific Phospho-SKI (Ser383) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SKI (Accession P12755), corresponding to amino acid residues around phosphorylated Ser383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816739 |
Product Specific Information
Antibody detects endogenous levels of SKI only when phosphorylated at Ser383.
Target Information
TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski also binds to Smad proteins specific to bone morphogenic proteins (BMPs) and blocks BMP signaling in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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