Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages, dendritic cells, and activated T cells.
CD80 binds with high affinity to CD28 and CD152 (CTLA-4), playing a key role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on Daudi cell line (5 µL/test, 0.5 µg/test)
• Excitation/Emission: 488 nm / 710 nm (Blue laser)
• Recommended Filter: 710/50 bandpass (695/40 acceptable)
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation.
CD80 is induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
CD80 dysfunction is associated with gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지