Thermo Fisher Scientific Complement C5b-9 Monoclonal Antibody (3R2/0)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3R2/0

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Purification

Protein A

Storage buffer

BBS

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2745462

Product Specific Information

This antibody recognizes the SC5b-9 complex of around 330 kDa. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex.

Target Information

The complement system is a fundamental part of innate immunity, consisting of over 45 genes that encode various complement components, regulators, and receptors. The complement C5b-9 complex, also known as the Membrane Attack Complex (MAC), plays a crucial role in the immune defense mechanism. It includes the sequential assembly of proteins C5b, C6, C7, C8, and multiple copies of C9. This complex forms transmembrane channels that puncture the cell membrane of target cells, leading to cell lysis. Specifically, MAC has significant implications in mediating cell death in both pathogenic organisms and cancer cells. The genes involved are clustered across the human genome, mainly organized in three significant loci. The understanding of the C5b-9 complex`s function extends beyond its antimicrobial roles to include its interactions with tumor cells, where it can induce cell death and influence cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.