Thermo Fisher Scientific NF45 Polyclonal Antibody
NF45 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. Human과 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 4°C 보관, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 340 and 390 of human Nuclear Factor of Activated T-cells 45 kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. tropicalis, and Orangutan
Target Information
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the IL-2 gene enhancer known as the antigen receptor response element 2. Additionally, NFAT can bind RNA and plays a key role in hepatitis B viral polymerase encapsidation and priming. NFAT exists as a heterodimer of 45 kDa and 90 kDa proteins, with the 45 kDa protein being the product of this gene, which binds strongly to the 90 kDa protein to enhance gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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