Thermo Fisher Scientific Phosphotyrosine (P-Tyr) Monoclonal Antibody (PY20)
Tyrosine 인산화 단백질 검출용 단클론 항체로 다양한 면역실험(WB, IHC, ICC, Flow, IP)에 사용 가능. Mouse IgG2b 형식으로 높은 특이성과 재현성을 제공. 4°C 보관, Protein A/G 정제, 연구용으로만 사용.
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Thermo Fisher Scientific Phosphotyrosine (P-Tyr) Monoclonal Antibody (PY20)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| Immunoprecipitation (IP) | 1–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PY20 |
| Immunogen | Phosphotyrosine conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tyrosine phosphorylation plays a critical role in signal transduction, mitogenesis, cell cycle progression, and oncogenic transformation. Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable for studying tyrosine-phosphorylated proteins and their biochemical pathways.
Clone PY20 anti-phosphotyrosine antibody is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical protocols, especially when secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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