Thermo Fisher Scientific XPA Monoclonal Antibody (5A2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:1,500

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 4 publications 4 publications

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5A2

Immunogen

Recombinant human XPA (Xeroderma Pigmentosum group A) protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_561966

Product Specific Information

Positive controls of LS174T, MCF-7 cells or human tonsil suggested. Works on pure and partially purified protein but not on crude cell extract.

Target Information

The XPA (xeroderma pigmentosumgroup A) protein specifically recognizes the UV-orchemically damaged DNA lesions, and triggers thenucleotide excision repair process. XPA binds to thereplication protein A (RPA) or the excision repaircross complementing 1 protein (ERCC 1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000 fold increased risk to develop skin cancer atsun-exposed areas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.