Thermo Fisher Scientific XPA Monoclonal Antibody (5A2)
XPA 단백질을 인식하는 Mouse IgG1 단클론 항체로, 인간 시료에 반응. WB, ELISA, IHC 등 다양한 응용 가능. 단백질 A 정제, 1.1 mg/mL 농도, PBS 용액 형태. UV 손상 DNA 복구 연구용으로 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:1,500 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View publication |
| ELISA | Assay-dependent | View 4 publications |
| Dot blot (DB) | Assay-dependent | View publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A2 |
| Immunogen | Recombinant human XPA (Xeroderma Pigmentosum group A) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_561966 |
Product Specific Information
Positive controls of LS174T, MCF-7 cells or human tonsil suggested. Works on pure and partially purified protein but not on crude cell extract.
Target Information
The XPA (xeroderma pigmentosum group A) protein specifically recognizes UV- or chemically damaged DNA lesions and triggers the nucleotide excision repair process. XPA binds to replication protein A (RPA) or excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair, persisting DNA lesions may lead to accumulation of mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000-fold increased risk of developing skin cancer at sun-exposed areas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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