Thermo Fisher Scientific RAD23B Polyclonal Antibody
Rabbit polyclonal antibody targeting human RAD23B protein. Suitable for WB, IHC, and IP applications. Provides high specificity and affinity via antigen affinity chromatography. Recommended for research use only.
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Thermo Fisher Scientific RAD23B Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 125 and 175 of Human UV excision repair protein RAD23 homolog (RAD23B, UniProt ID: P54727-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The RAD23B protein is one of two human homologs of Saccharomyces cerevisiae Rad23, involved in nucleotide excision repair (NER). It complements the NER defect in xeroderma pigmentosum group C (XP-C) cell extracts and interacts with 3-methyladenine-DNA glycosylase (MPG), suggesting a role in DNA damage recognition during base excision repair. It contains an N-terminal ubiquitin-like domain that interacts with the 26S proteasome, indicating involvement in ubiquitin-mediated proteolysis.
Important Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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