Thermo Fisher Scientific Ubiquitin Polyclonal Antibody

Applications

• Western Blot (WB): Tested dilution 1:500–1:10,000

Product Specifications

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial.
For short-term storage (1–2 weeks), store at 4°C.
For long-term storage, aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.

Target Information

Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
This gene encodes a fusion protein consisting of ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S ribosomal subunit, belongs to the S27AE family, contains C4-type zinc finger domains, and is located in the cytoplasm.
Pseudogenes derived from this gene are present in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.