Thermo Fisher Scientific NGAL Monoclonal Antibody (17)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

17

Immunogen

Recombinant dog NGAL adsorbed onto aluminum hyroxide gel.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.10 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536225

Product Specific Information

ABS 047-17-02 binds free canine NGAL.

This antibody will not cross-react with human, mouse, or rat. ABS 047-17-02 can be used as a capture antibody in a sandwich ELISA using biotinylated detection antibody ABS 047-14-02, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.