Thermo Fisher Scientific NGAL Monoclonal Antibody (17)
개 및 NGAL 단백질 특이적 단클론 항체로, Western blot, IHC, ELISA에 사용 가능. 인간, 마우스, 랫과 교차 반응 없음. 단백질 A/G 정제, PBS 완충액에 4°C 보관. ELISA 캡처 항체로 5 pg/mL 감지 한계.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17 |
| Immunogen | Recombinant dog NGAL adsorbed onto aluminum hydroxide gel |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.10 mg/mL (lot-dependent; 0.85–1.15 mg/mL) |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536225 |
Product Specific Information
- ABS 047-17-02 binds free canine NGAL.
- No cross-reactivity with human, mouse, or rat.
- Can be used as a capture antibody in a sandwich ELISA with biotinylated detection antibody ABS 047-14-02.
- Detection limit: 5 pg/mL in an unoptimized buffer assay.
- Concentration may vary by lot (0.85–1.15 mg/mL).
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, also known as lipocalin 2, siderocalin, or neutrophil lipocalin) is a 25 kDa glycosylated monomer of the lipocalin family that binds and transports small lipophilic molecules. It is released by activated neutrophils and can form dimers or higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B).
Low-level NGAL expression in various epithelia may increase during inflammation or cancer. Expression in the kidney is markedly elevated by ischemic or nephrotoxic injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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