Thermo Fisher Scientific beta Actin Loading Control Monoclonal Antibody (BA3R)
베타 액틴을 표적하는 단클론 항체로, Western blot 및 IHC 등 다양한 응용에 적합합니다. 인간, 마우스, 랫 등 여러 종에 반응하며, 단백질 적재 대조군으로 널리 사용됩니다. 비결합형 형태로 제공되며, 고순도 Protein A 정제 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | 331 publications |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 15 publications |
| Flow Cytometry (Flow) | 1–5 µg/1×10⁶ cells | 1 publication |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | – | 2 publications |
| Inhibition Assays (IA) | – | 1 publication |
| Miscellaneous PubMed (Misc) | – | 6 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rabbit, Rat |
| Published Species | Chicken, Fungi, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rabbit, Rat, Xenopus |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BA3R |
| Immunogen | Beta-actin N-terminal peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979409 |
Available Conjugated Formats
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- HRP
- Request custom conjugation
Product Specific Information
Storage and handling: Add 0.05% sodium azide if desired. For long-term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein present in all human cell types and plays essential roles in cell motility, structure, and integrity.
Beta and gamma actins coexist in most cells as cytoskeletal components. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) with a two-stranded helical structure.
Due to its ubiquitous expression, beta actin is commonly used as a loading control in protein detection assays such as Western blotting.
Antibodies against beta-actin are valuable tools for studying intracellular distribution and cytoskeletal organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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