Thermo Fisher Scientific Nesprin 1 Recombinant Rabbit Monoclonal Antibody (1D4D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1D4D9

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 8200-8400 of human Nesprin 1 (Q8NF91). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nesprin 1,uniProtId:Q8NF91-1,ncbiNodeId:9606,antigenRange:8200-8400,antigenLength:8797,antigenImageFileName:MA5-42738_Nesprin_1_Q8NF91-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42738_Nesprin_1_Q8NF91-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911879

Product Specific Information

Immunogen sequence: FGRVERYHKK LIRLPLPDDE HDLSDRELEL EDSAALSDLH WHDRSADSLL SPQPSSNLSL SLAQPLRSER SGRDTPASVD SIPLEWDHDY DLSRDLESAM SRALPSEDEE GQDDKDFYLR GAVGLSGDHS ALESQIRQLG KALDDSRFQI QQTENIIRSK TPTGPELDTS YKGYMKLLGE CSSSIDSVKR LEHKLKEEEE S

Target Information

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-l and -2 for nuclear _envelope _spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structura integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.